Skip to content
GitLab
Explore
Sign in
Primary navigation
Search or go to…
Project
O
openbis
Manage
Activity
Members
Labels
Code
Merge requests
Repository
Branches
Commits
Tags
Repository graph
Compare revisions
Snippets
Build
Pipelines
Jobs
Pipeline schedules
Artifacts
Deploy
Releases
Model registry
Analyze
Value stream analytics
Contributor analytics
CI/CD analytics
Repository analytics
Help
Help
Support
GitLab documentation
Compare GitLab plans
Community forum
Contribute to GitLab
Provide feedback
Keyboard shortcuts
?
Snippets
Groups
Projects
Show more breadcrumbs
sispub
openbis
Commits
57714660
Commit
57714660
authored
15 years ago
by
tpylak
Browse files
Options
Downloads
Patches
Plain Diff
SE-209 screening: how to import screen data into openBIS
SVN: 14842
parent
c536a387
No related branches found
Branches containing commit
No related tags found
Tags containing commit
No related merge requests found
Changes
1
Hide whitespace changes
Inline
Side-by-side
Showing
1 changed file
screening_tools/dist/readme.txt
+26
-8
26 additions, 8 deletions
screening_tools/dist/readme.txt
with
26 additions
and
8 deletions
screening_tools/dist/readme.txt
+
26
−
8
View file @
57714660
High Content Screening: how to import a library into openBIS
-------------------
High Content Screening: how to import screen data into openBIS
I. Transform the library file to adhere to openBIS format
------
To transform a QIAGEN library (or a file in similar format) you can use the command line tool called "metadata importer".
It takes following parameters:
...
...
@@ -14,16 +15,33 @@ e.g:
metadata-importer.bat master-plate-example.csv /LMC/BLUE-PROJECT/TRAIL-EXPERIMENT 384_WELLS_16X24 LMC
The result of the transformation are 3 files, for genes, oligos and plates.
You have to register these files manually from the web browser in openBIS in following order:
- genes: go to "Material -> Import" menu and select material type "GENE". Then select a genes.txt file and click "Save".
- oligos: as above but select material type "OLIGO"
- plates: go to "Sample -> Import" and select sample type "(multiple)" - you will register plates and wells at the same time.
You have to register these files manually from the web browser in openBIS (see next steps).
-----
Note for Java developers:
Note for Java developers:
If your library file format is slightly different you can get the "metadata importer" source code and modify it to fit your needs.
The source code is available at:
https://svncisd.ethz.ch/repos/cisd/screening_tools/trunk
You are interested in this package:
ch.systemsx.cisd.openbis.metadata
You can build the package using ant 'metadata-jar' target (build/build.xml).
-----
\ No newline at end of file
II. Register genes and oligos
------
1. Register genes: go to "Material -> Import" menu and select material type "GENE". Then select a genes.txt file and click "Save".
2. Register oligos: as above but select material type "OLIGO"
III. Register the experiment with plates
------
1. Ensure that the appropriate group and project exist
- you can create a new group in "Administration -> Groups", "Add group"
- you can create a new project in "Administration -> Project -> New"
2. Register the experiment with the plates
Go to "Experiment -> New"
- specify the code of your experiment and choose a project for it
- select "Samples: register from a file and attach"
- for "Sample Type" choose "(multiple)"
- choose a "plates.txt" file generated in the step I.
- fill other atributes and click "Save".
It will take some time to register an experiment if you have a lot of plates in the file.
This diff is collapsed.
Click to expand it.
Preview
0%
Loading
Try again
or
attach a new file
.
Cancel
You are about to add
0
people
to the discussion. Proceed with caution.
Finish editing this message first!
Save comment
Cancel
Please
register
or
sign in
to comment