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Commit 2492881c authored by Adam Laskowski's avatar Adam Laskowski
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BIS-1034: removed illumina_ngs from installation options

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...@@ -65,9 +65,6 @@ ...@@ -65,9 +65,6 @@
<field type="check" variable="FLOW"> <field type="check" variable="FLOW">
<spec txt="Flow Cytometry" true="true" false="false"/> <spec txt="Flow Cytometry" true="true" false="false"/>
</field> </field>
<field type="check" variable="ILLUMINA-NGS">
<spec txt="Illumina NGS (ETH BSSE Setup)" true="true" false="false"/>
</field>
</panel> </panel>
<panel id="UserInputPanel.MISC"> <panel id="UserInputPanel.MISC">
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...@@ -35,9 +35,6 @@ KEY_STORE_PASSWORD = changeit ...@@ -35,9 +35,6 @@ KEY_STORE_PASSWORD = changeit
# Password of the key # Password of the key
KEY_PASSWORD = changeit KEY_PASSWORD = changeit
# Standard technology ILLUMINA-NGS (ETH BSSE Setup) is disabled by default
#ILLUMINA-NGS = true
# Standard technology ELN-LIMS is disabled by default # Standard technology ELN-LIMS is disabled by default
#ELN-LIMS = true #ELN-LIMS = true
......
...@@ -43,8 +43,6 @@ public class GlobalInstallationContext ...@@ -43,8 +43,6 @@ public class GlobalInstallationContext
public static final String BACKUP_FOLDER_VARNAME = "BACKUP_FOLDER"; public static final String BACKUP_FOLDER_VARNAME = "BACKUP_FOLDER";
public static final String TECHNOLOGY_ILLUMINA_NGS = "ILLUMINA-NGS";
public static final String TECHNOLOGY_ELN_LIMS = "ELN-LIMS"; public static final String TECHNOLOGY_ELN_LIMS = "ELN-LIMS";
public static final String TECHNOLOGY_ELN_LIMS_TEMPLATE_TYPES = "ELN-LIMS-TEMPLATE-TYPES"; public static final String TECHNOLOGY_ELN_LIMS_TEMPLATE_TYPES = "ELN-LIMS-TEMPLATE-TYPES";
...@@ -56,7 +54,7 @@ public class GlobalInstallationContext ...@@ -56,7 +54,7 @@ public class GlobalInstallationContext
public static final String TECHNOLOGY_SHARED_MICROSCOPY_FLOW_CYTOMETRY = "SHARED"; public static final String TECHNOLOGY_SHARED_MICROSCOPY_FLOW_CYTOMETRY = "SHARED";
public static final String[] TECHNOLOGIES = public static final String[] TECHNOLOGIES =
{ TECHNOLOGY_ILLUMINA_NGS, TECHNOLOGY_ELN_LIMS, TECHNOLOGY_MICROSCOPY, { TECHNOLOGY_ELN_LIMS, TECHNOLOGY_MICROSCOPY,
TECHNOLOGY_FLOW_CYTOMETRY, TECHNOLOGY_ELN_LIMS_TEMPLATE_TYPES }; TECHNOLOGY_FLOW_CYTOMETRY, TECHNOLOGY_ELN_LIMS_TEMPLATE_TYPES };
/** /**
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...@@ -71,74 +71,6 @@ ...@@ -71,74 +71,6 @@
<p></p> <p></p>
<h4 class="illumina-ngs">Illumina NGS (ETH BSSE Setup)</h4>
<ol>
<li>A flow cell with flow lanes is created reading out two XML files provided by the Illumina sequencer:
<div class="sourcecode">
mkdir -p ${DSS_ROOT_DIR}/create-flowcell-hiseq/120420_SN792_0109_BC0P8LACXX/;<br>
cp -R ${DSS_ROOT_DIR}/examples/illumina-ngs/120420_SN792_0109_BC0P8LACXX/*.xml
${DSS_ROOT_DIR}/create-flowcell-hiseq/120420_SN792_0109_BC0P8LACXX;<br>
touch ${DSS_ROOT_DIR}/create-flowcell-hiseq/.MARKER_is_finished_120420_SN792_0109_BC0P8LACXX<br>
</div>
</li>
<li>A flow cell output from a HiSeq2000 is registered as a data set of the object 120420_SN792_0109_BC0P8LACXX.<br>
Additionally a data set is registered for each flow lane:
<div class="sourcecode">
rsync -a --exclude=Unaligned* ${DSS_ROOT_DIR}/examples/illumina-ngs/120420_SN792_0109_BC0P8LACXX
${DSS_ROOT_DIR}/register-flowcell-hiseq;<br>
touch ${DSS_ROOT_DIR}/register-flowcell-hiseq/.MARKER_is_finished_120420_SN792_0109_BC0P8LACXX;<br>
</div>
</li>
<li>The sequencer is finished and we want to track this automatically. Therefore we sync the RTAComplete.txt in a
drop box:
<div class="sourcecode">
mkdir -p ${DSS_ROOT_DIR}/read-rta-timestamp/120420_SN792_0109_BC0P8LACXX/; rsync
${DSS_ROOT_DIR}/examples/illumina-ngs/120420_SN792_0109_BC0P8LACXX/RTAComplete.txt
${DSS_ROOT_DIR}/read-rta-timestamp/120420_SN792_0109_BC0P8LACXX/RTAComplete.txt; touch
${DSS_ROOT_DIR}/read-rta-timestamp/.MARKER_is_finished_120420_SN792_0109_BC0P8LACXX
</div>
</li>
<li> For creating some objects we use the 'Object Registration'. This is available under the 'Import' menu: <br>
Import -> Object Registration -> Choose object type '(multiple)' and browse to the file:
<div class="sourcecode"> ${DSS_ROOT_DIR}/examples/illumina-ngs/tsv-files/example_samples_with_parents.tsv</div>
Tick 'Update existing'and press the 'Save' button. <br>
This created several objects which can be viewed via Browse -> Objects. You might need to select the Object Type 'all' <br>
and the Space 'all'. <br>
Note that the objects are in a parent-/child relationship indicated by the objects shown in the 'Parents' column.
</li>
<br>
<li>After the demultiplexing using the Illumina 'configureBclToFastq.pl' the FASTQ files are created and also some
statistic files are provided.<br>
Let's register them as well in openBIS:
<div class="sourcecode">
mkdir ${DSS_ROOT_DIR}/register-unaligned/120420_SN792_0109_BC0P8LACXX; <br>
rsync -a ${DSS_ROOT_DIR}/examples/illumina-ngs/120420_SN792_0109_BC0P8LACXX/Unaligned* ${DSS_ROOT_DIR}/register-unaligned/120420_SN792_0109_BC0P8LACXX;<br>
touch ${DSS_ROOT_DIR}/register-unaligned/.MARKER_is_finished_120420_SN792_0109_BC0P8LACXX;
</div>
<br>
The demultiplexed FASTQ files are a data set of the objects BSSE-QGF-LIBRARY-1 and BSSE-QGF-LIBRARY-2. Note that lane 1 and two <br>
contained both objects which results in two FASTQ data sets for each object.
</li>
</ol><br>
A short description of all drop boxes is also provided here: <a href=
"https://wiki-bsse.ethz.ch/display/openBISDoc/openBIS+for+Illumina+NGS" target="_blank">openBIS for Illumina
NGS<a><br>
<h4 class="eln-lims">ELN-LIMS</h4> <h4 class="eln-lims">ELN-LIMS</h4>
<p>Here are the steps to create your first experimental step and register an image file as preview into the ELN-LIMS interface.</p> <p>Here are the steps to create your first experimental step and register an image file as preview into the ELN-LIMS interface.</p>
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