data-set-handler.py

#! /usr/bin/env python
# This is an example Jython dropbox for importing HCS image datasets

import os
import shutil
import random

import ch.systemsx.cisd.openbis.generic.shared.basic.dto as dto
from ch.systemsx.cisd.openbis.generic.shared.basic.dto import SampleType, NewSample
from ch.systemsx.cisd.openbis.generic.shared.dto.identifier import SampleIdentifier
from ch.systemsx.cisd.openbis.dss.etl.dto.api.v1 import *
from ch.systemsx.cisd.openbis.dss.etl.custom.geexplorer import GEExplorerImageAnalysisResultParser
from java.io import File

from ch.systemsx.cisd.common.fileconverter import FileConverter, Tiff2PngConversionStrategy
from ch.systemsx.cisd.common.mail import From

# ------------
# Dropbox specific image dataset registration. You may want to modify this part.
# ------------

""" type of the new image dataset """
IMAGE_DATASET_TYPE = "HCS_IMAGE"
""" file format code of files in a new image dataset """
IMAGE_FILE_FORMAT = "TIFF"

""" type of the new analysis dataset """
ANALYSIS_DATASET_TYPE = "HCS_IMAGE_ANALYSIS_DATA"
""" file format of the analysis dataset """
ANALYSIS_FILE_FORMAT = "CSV"

""" type of the new image overlay dataset """
OVERLAY_IMAGE_DATASET_TYPE = "HCS_IMAGE_SEGMENTATION_OVERLAY"
""" file format of the image overlay dataset """
OVERLAY_IMAGE_FILE_FORMAT = "PNG"

""" space where the plate for which the dataset has been acquired exist """
PLATE_SPACE = "DEMO"

""" only files with these extensions will be recognized as images """
RECOGNIZED_IMAGES_EXTENSIONS = ["tiff", "tif", "png", "gif", "jpg", "jpeg"]

# ---------

""" sample type code of the plate, needed if a new sample is registered automatically """
PLATE_TYPE_CODE = "PLATE"
""" project and experiment where new plates will be registered """
DEFAULT_PROJECT_CODE = "TEST"
DEFAULT_EXPERIMENT_CODE = "SANOFI"
PLATE_GEOMETRY_PROPERTY_CODE = "$PLATE_GEOMETRY"
PLATE_GEOMETRY = "384_WELLS_16X24"

# ---------

""" extracts code of the sample from the directory name """
def extract_sample_code(incoming_name):
    file_basename = extract_file_basename(incoming_name)
    #return file_basename.split(".")[0]
    code = file_basename[file_basename.find("plates_") + 7 : file_basename.rfind("_") ]
    if code == "":
        code = file_basename
    return code

""" 
For a given tile number and tiles geometry returns a (x,y) tuple which describes where the tile
is located on the well.
"""
def get_tile_coords(tile_num, tile_geometry):
    columns = tile_geometry[1]
    row = ((tile_num - 1) / columns) + 1
    col = ((tile_num - 1) % columns) + 1
    return (row, col)

""" 
Parameters:
    image_tokens_list - list of ImageTokens
Returns:  (rows, columns) tuple describing the matrix of tiles (aka fields or sides) in the well  
"""
def get_tile_geometry(image_tokens_list):
    max_tile = get_max_tile_number(image_tokens_list)
    if max_tile % 4 == 0 and max_tile != 4:
        return (max_tile / 4, 4)
    elif max_tile % 3 == 0:
        return (max_tile / 3, 3)
    elif max_tile % 2 == 0:
        return (max_tile / 2, 2)
    else:
        return (max_tile, 1)

"""
Creates ImageFileInfo for a given ImageTokens.
Converts tile number to coordinates on the 'well matrix'.
Example file name: A - 1(fld 1 wv Cy5 - Cy5).tif
Returns:
    ImageTokens
"""
def create_image_tokens(path):
    image_tokens = ImageTokens()
    image_tokens.path = path

    basename = os.path.splitext(path)[0]

    wellText = basename[0:find(basename, "(")] # A - 1
    image_tokens.well = wellText.replace(" - ", "")
    
    fieldText = basename[find(basename, "fld ") + 4 : find(basename, " wv")]
    try:
        image_tokens.tile = int(fieldText)
        #print "image_tokens.tile", image_tokens.tile
    except ValueError:
        raise Exception("Cannot parse field number from '" + fieldText + "' in '" + basename + "' file name.")

    image_tokens.channel = basename[rfind(basename, " - ") + 3 :-1]
    return image_tokens

# ------------
# END of the part which you will probably need to modify
# ------------

# ------------
# Generic utility
# ------------

""" 
Finds first occurence of the patter from the right.
Throws exception if the pattern cannot be found.
"""
def rfind(text, pattern):
    ix = text.rfind(pattern)
    ensurePatternFound(ix, text, pattern)
    return ix

""" 
Finds first occurence of the patter from the left. 
Throws exception if the pattern cannot be found.
"""
def find(text, pattern):
    ix = text.find(pattern)
    ensurePatternFound(ix, text, pattern)
    return ix

def ensurePatternFound(ix, file, pattern):
    if ix == -1:
        raise Exception("Cannot find '" + pattern + "' pattern in file name '" + file + "'")    

""" Returns: name of the file without the extension """
def extract_file_basename(filename):
    lastDot = filename.rfind(".")
    if lastDot != -1:
        return filename[0:lastDot]
    else:
        return filename

""" Returns: extension of the file """
def get_file_ext(file):
    return os.path.splitext(file)[1][1:].lower()

""" Returns: java.io.File - first file with the specified extension or None if no file matches """
def find_file_by_ext(incoming_file, expected_ext):
    if not incoming_file.isDirectory():
        return None
    incoming_path = incoming_file.getPath()
    for file in os.listdir(incoming_path):
        ext = get_file_ext(file)
        if ext.upper() == expected_ext.upper():
            return File(incoming_path, file)
    return None

""" Returns: java.io.File - subdirectory which contains the specified marker in the name """
def find_dir(incoming_file, dir_name_marker):
    if not incoming_file.isDirectory():
        return None
    incoming_path = incoming_file.getPath()
    for file in os.listdir(incoming_path):
        if dir_name_marker.upper() in file.upper():
            return File(incoming_path, file)
    return None

def get_random_string():
    return str(int(random.random()*1000000000))

""" 
Creates a temporary directory two levels above the specified incoming file.
The name of the directory will contain the specified label and a random text. 
Returns:
    java.io.File - path to the temporary directory
"""
def get_tmp_dir(incoming, label):
    dropbox_parent_dir = incoming.getParentFile().getParent()
    tmp_dir = File(dropbox_parent_dir, "tmp")
    if not os.path.exists(tmp_dir.getPath()):
        os.mkdir(tmp_dir.getPath())
    tmp_labeled_dir = File(tmp_dir, label + ".tmp." + get_random_string())
    os.mkdir(tmp_labeled_dir.getPath())
    return tmp_labeled_dir

# ------------
# Image dataset registration
# ------------

"""
Auxiliary function to extract all channel codes used by specified images.
The channel label will be equal to channel code.
Parameters:
    images - list of ImageFileInfo
Returns: 
    list of Channel
"""
def get_available_channels(images):
    channel_codes = {}
    for image in images:
        channel_codes[image.getChannelCode()] = 1
    channels = []
    for channelCode in channel_codes.keys():
        channels.append(Channel(channelCode, channelCode))
    return channels

"""
Parameters:
    dataset - BasicDataSetInformation
    registration_details - DataSetRegistrationDetails
"""
def set_dataset_details(dataset, registration_details):
    registration_details.setDataSetInformation(dataset)
    registration_details.setFileFormatType(dataset.getFileFormatTypeCode())
    registration_details.setDataSetType(dataset.getDataSetType())
    registration_details.setMeasuredData(dataset.isMeasured())

"""
Parameters:
    dataset - BasicDataSetInformation
Returns: 
    DataSetRegistrationDetails
"""
def create_image_dataset_details(incoming):
    registration_details = factory.createImageRegistrationDetails()
    image_dataset = registration_details.getDataSetInformation()
    set_image_dataset(incoming, image_dataset)

    set_dataset_details(image_dataset, registration_details)
    return registration_details


""" Returns: integer - maximal tile number """
def get_max_tile_number(image_tokens_list):
    max_tile = 0
    for image_tokens in image_tokens_list:
        max_tile = max(max_tile, image_tokens.tile)
    return max_tile

""" Auxiliary structure to store tokens of the image file name.  """
class ImageTokens:
    # channel code
    channel = None
    # tile number
    tile = -1
    # path to the image
    path = ""
    # well code, e.g. A1
    well = ""

""" 
Creates ImageFileInfo for a given path to an image
Example of the accepted file name: A - 1(fld 1 wv Cy5 - Cy5).tif
Returns:
   ImageFileInfo 
"""
def create_image_info(image_tokens, tile_geometry):
    tileCoords = get_tile_coords(image_tokens.tile, tile_geometry)
    img = ImageFileInfo(image_tokens.channel, tileCoords[0], tileCoords[1], image_tokens.path)
    img.setWell(image_tokens.well)
    return img

"""
Tokenizes file names of all images in the directory.
Returns: 
  list of ImageTokens
"""
def parse_image_tokens(dir, recognized_image_extensions):
    image_tokens_list = []
    dir_path = dir.getPath()
    for file in os.listdir(dir_path):
        ext = get_file_ext(file)
        try:
            extIx = recognized_image_extensions.index(ext)
            # not reached if extension not found
            image_tokens = create_image_tokens(file)
            #print "tile", image_tokens.tile, "path", image_tokens.path, "well", image_tokens.well
            image_tokens_list.append(image_tokens)    
        except ValueError:
            pass # extension not recognized    
    return image_tokens_list

"""
Parameters:
- image_tokens_list - list of ImageTokens for each image
- tile_geometry - (rows, columns) tuple describing the matrix of tiles (aka fields or sides) in the well  
Returns: 
  list of ImageFileInfo
"""    
def create_image_infos(image_tokens_list, tile_geometry):
    images = []
    for image_tokens in image_tokens_list:
        image = create_image_info(image_tokens, tile_geometry)
        images.append(image)    
    return images

# ---------------------

"""
Extracts all images from the incoming directory.
Parameters:
    incoming - java.io.File, folder with images
    dataset - ImageDataSetInformation where the result will be stored
"""
def set_image_dataset(incoming, dataset):
    dataset.setDatasetTypeCode(IMAGE_DATASET_TYPE)
    dataset.setFileFormatCode(IMAGE_FILE_FORMAT)

    sample_code = extract_sample_code(incoming.getName())
    dataset.setSample(PLATE_SPACE, sample_code)
    dataset.setMeasured(True)

    image_tokens_list = parse_image_tokens(incoming, RECOGNIZED_IMAGES_EXTENSIONS)
    tile_geometry = get_tile_geometry(image_tokens_list)
    images = create_image_infos(image_tokens_list, tile_geometry)
    channels = get_available_channels(images)
    
    dataset.setImages(images)
    dataset.setChannels(channels)
    dataset.setTileGeometry(tile_geometry[0], tile_geometry[1])

    return dataset

"""
Extracts all overlay images from the overlays_dir directory.
Parameters:
    overlays_dir - java.io.File, folder with 
    image_dataset - ImageDataSetInformation, image dataset to which the overlay dataset belongs
    img_dataset_code - string, code of the  image dataset to which the overlay dataset belongs
    overlay_dataset - ImageDataSetInformation where the result will be stored
    extension - accepted image file extensions
"""
def set_overlay_dataset(overlays_dir, image_dataset, img_dataset_code, overlay_dataset, extension):
    overlay_dataset.setDatasetTypeCode(OVERLAY_IMAGE_DATASET_TYPE)
    overlay_dataset.setFileFormatCode(OVERLAY_IMAGE_FILE_FORMAT)

    overlay_dataset.setSample(image_dataset.getSpaceCode(), image_dataset.getSampleCode())
    overlay_dataset.setMeasured(False)
    overlay_dataset.setParentDatasetCode(img_dataset_code)

    if extension == None:
        recognized_image_exts = RECOGNIZED_IMAGES_EXTENSIONS
    else:
        recognized_image_exts = [ extension ]
    image_tokens_list = parse_image_tokens(overlays_dir, recognized_image_exts)
    tile_geometry = (image_dataset.getTileRowsNumber(), image_dataset.getTileColumnsNumber())
    images = create_image_infos(image_tokens_list, tile_geometry)
    channels = get_available_channels(images)

    overlay_dataset.setImages(images)
    overlay_dataset.setChannels(channels)
    overlay_dataset.setTileGeometry(tile_geometry[0], tile_geometry[1])

"""
Creates registration details of the image overlays dataset.
Parameters:
    overlays_dir - java.io.File, folder with 
    image_dataset - ImageDataset, image dataset to which the overlay dataset belongs
    img_dataset_code - string, code of the  image dataset to which the overlay dataset belongs
Returns:
    DataSetRegistrationDetails
"""
def create_overlay_dataset_details(overlays_dir, image_dataset, img_dataset_code, extension):
    overlay_dataset_details = factory.createImageRegistrationDetails()
    overlay_dataset = overlay_dataset_details.getDataSetInformation()
    set_overlay_dataset(overlays_dir, image_dataset, img_dataset_code, overlay_dataset, extension)
    set_dataset_details(overlay_dataset, overlay_dataset_details)

    config = ImageStorageConfiguraton.createDefault()
    # channels will be connected to the dataset
    config.setStoreChannelsOnExperimentLevel(False)
    overlay_dataset.setImageStorageConfiguraton(config)
    return overlay_dataset_details

# ---------------------

"""
Creates the analysis dataset description. 
The dataset will be connected to the specified sample and parent dataset.
Parameters:
    dataset - BasicDataSetInformation where the result will be stored
"""
def set_analysis_dataset(sample_space, sample_code, parent_dataset_code, dataset):
    dataset.setDatasetTypeCode(ANALYSIS_DATASET_TYPE)
    dataset.setFileFormatCode(ANALYSIS_FILE_FORMAT)
    dataset.setSample(sample_space, sample_code)
    dataset.setMeasured(False)
    dataset.setParentDatasetCode(parent_dataset_code)

"""
Creates registration details of the analysis dataset.
Returns:
    DataSetRegistrationDetails
"""
def create_analysis_dataset_details(sample_space, sample_code, parent_dataset_code):
    registration_details = factory.createBasicRegistrationDetails()
    dataset = registration_details.getDataSetInformation()
    set_analysis_dataset(sample_space, sample_code, parent_dataset_code, dataset)
    set_dataset_details(dataset, registration_details)
    return registration_details

""" registers sample if it does not exist already """
def register_sample_if_necessary(space_code, project_code, experiment_code, sample_code):   
    openbis = state.getOpenBisService()
    sampleIdentifier = SampleIdentifier.create(space_code, sample_code)
    if (openbis.tryGetSampleWithExperiment(sampleIdentifier) == None):
        sample = NewSample()
        sampleType = SampleType()
        sampleType.setCode(PLATE_TYPE_CODE)
        sample.setSampleType(sampleType)
        sample.setIdentifier(sampleIdentifier.toString())
        
        property = dto.VocabularyTermEntityProperty();
        vocabularyTerm = dto.VocabularyTerm();
        vocabularyTerm.setCode(PLATE_GEOMETRY);
        property.setVocabularyTerm(vocabularyTerm);
        propertyType = dto.PropertyType();
        dataType = dto.DataType();
        dataType.setCode(dto.DataTypeCode.CONTROLLEDVOCABULARY);
        propertyType.setDataType(dataType);
        propertyType.setCode(PLATE_GEOMETRY_PROPERTY_CODE);
        property.setPropertyType(propertyType);
        sample.setProperties([ property ])
        
        sample.setExperimentIdentifier("/" + space_code + "/" + project_code + "/" + experiment_code)
        openbis.registerSample(sample, None)

# ---------------------

def debug(*msg):
    print "".join(msg)
    
def convert_to_png(dir, transparent_color):
    strategy = Tiff2PngConversionStrategy(transparent_color)
    # Uses #cores * machineLoad threads for the conversion, but not more than maxThreads
    machineLoad = 1
    maxThreads = 4
    status = FileConverter.performConversion(File(dir), strategy, machineLoad, maxThreads)
    errorMsg = FileConverter.tryFailuresToString(status)
    if errorMsg != None:
        raise Exception("Error", errorMsg)

def notify(plate_code):
    content  = "Dear Mr./Mrs.\n"
    hostname = "http://bwl27.sanofi-aventis.com:8443/openbis"
    plate_link = "<a href="+hostname+"#entity=SAMPLE&action=SEARCH&code="+plate_code+">"+plate_code+"</a>"
    content += "Data for the plate " + plate_link + " has been registered.\n"
    content += "\n"
    content += "Have a nice day!\n"
    content += "   openBIS\n"
    replyAddress = "Matthew.Smicker@sanofi-aventis.com"
    fromAddress = From("openbis@sanofi-aventis.com")
    recipients = [ "Matthew.Smicker@sanofi-aventis.com", "tpylak@ethz.ch" ] 
    state.mailClient.sendMessage("openBIS: registration finished", content, replyAddress, fromAddress, recipients)

       
"""
Allows to recognize that the subdirectory of the incoming dataset directory contains overlay images.
This text has to appear in the subdirectory name. 
"""
OVERLAYS_DIR_PATTERN = "_ROITiff"

def register_images_with_overlays_and_analysis(incoming):
    if not incoming.isDirectory():
        return
    
    tr = service.transaction(incoming, factory)
        
    image_dataset_details = create_image_dataset_details(incoming)
    plate_code = image_dataset_details.getDataSetInformation().getSampleCode()
    space_code = image_dataset_details.getDataSetInformation().getSpaceCode()
    register_sample_if_necessary(space_code, DEFAULT_PROJECT_CODE, DEFAULT_EXPERIMENT_CODE, plate_code)

    # create the image data set and put everything in it initially
    image_data_set = tr.createNewDataSet(image_dataset_details)
    image_data_set_folder = tr.moveFile(incoming.getPath(), image_data_set)
    img_dataset_code = image_data_set.getDataSetCode()
          
        
    # move overlays folder
    overlays_dir = find_dir(File(image_data_set_folder), OVERLAYS_DIR_PATTERN)
    if overlays_dir != None:
        convert_to_png(overlays_dir.getPath(), "white")
        overlay_dataset_details = create_overlay_dataset_details(overlays_dir, 
                                     image_dataset_details.getDataSetInformation(), img_dataset_code, "png")
        overlays_data_set = tr.createNewDataSet(overlay_dataset_details)
        tr.moveFile(overlays_dir.getPath(), overlays_data_set, "overlays")

    # transform and move analysis file
    analysis_file = find_file_by_ext(File(image_data_set_folder), "xml")
    if analysis_file != None:
        analysis_registration_details = create_analysis_dataset_details(space_code, plate_code, img_dataset_code)
        analysis_data_set = tr.createNewDataSet(analysis_registration_details)
        analysis_data_set_file = tr.createNewFile(analysis_data_set, analysis_file.getName())
        GEExplorerImageAnalysisResultParser(analysis_file.getPath()).writeCSV(File(analysis_data_set_file))
        
    service.commit()
    notify(plate_code)
    
register_images_with_overlays_and_analysis(incoming)