@@ -118,7 +118,7 @@ Moreover, you may only be interested in a subset of the cells visible in the ima
When you want to extract fluorescence, you can add files from which to extract fluorescence by clicking the `Add` button. There you can either add a single image file, a multistack TIFF file, or a folder containing image files. Note that if multiple files or frames are used, the fluorescence file or folder must have the same amount of images as the image given to the program at startup.
The output csv contains one line for every combination of cells, timeframe, and channel. This allows the file easily to be read into pandas, and avoids a varying amount of columns depending on how many fluorescence channels are used. The following statistics are exported: The *area* of the cell, the *mean* intensity, the intensity *variance*, the *total intensity*, and the x and y coordinates of the *center of mass*. Moreover, the major and minor axes of the cells are found using principal component analysis. In particular, the *angle* of the major axis to the x axis is given, together with the *length* of the major and minor axis, thus, fully specifying an ellipsoid approximatiing the cell. Finally, we report whether the cell disappears, i.e., whether or not it is present in the last frame.
The output csv contains one line for every combination of cells, timeframe, and channel. This allows the file easily to be read into pandas, and avoids a varying amount of columns depending on how many fluorescence channels are used. The following statistics are exported: The *area* of the cell, the *mean* intensity, the intensity *variance*, the *total intensity*, and the x and y coordinates of the *center of mass*. Moreover, the major and minor axes of the cells are found using principal component analysis. In particular, the *angle* of the major axis to the x axis is given, together with the *length* of the major and minor axis, thus, fully specifying an ellipsoid approximating the cell. Finally, we report whether the cell disappears, i.e., whether or not it is present in the last frame.
