diff --git a/init/InitButtons.py b/init/InitButtons.py
index 3848d855541b874f04bb054393d5077f11e3e6b8..7967853f32f966359345415eddf0f5980ada5fd0 100644
--- a/init/InitButtons.py
+++ b/init/InitButtons.py
@@ -2,310 +2,250 @@
 """
 Initializing all the buttons in this file.
 """
-from PyQt5.QtWidgets import QWidget, QPushButton, QShortcut, QComboBox, QCheckBox, QLineEdit, QStatusBar
+from PyQt5.QtWidgets import QPushButton, QComboBox, QLineEdit
 from PyQt5 import QtGui
-from PyQt5.QtCore import pyqtSignal, QObject, Qt
+from PyQt5.QtCore import Qt
 import numpy as np
 
 def Init(parent):
+    """
+    configuration of all the buttons, some buttons just need to be toggled
+    meaning that they need just to be clicked and the function associated 
+    to the button is called and executed. Other buttons are checkable 
+    meaning that until the user has not finished to use the function
+    connected to the button, this button stays active (or Checked)
+    """
+
+    # hide / show cnn buttons
+    parent.button_hide_show.setCheckable(True)    
+    parent.button_hide_show.setMaximumWidth(150)
+    parent.button_hide_show.clicked.connect(parent.ShowHideCNNbuttons)
+    parent.button_hide_show.setStatusTip('Press to hide and show neural network buttons')
+
+    # ADD REGION
+    parent.button_add_region.setCheckable(True)
+    parent.button_add_region.setMaximumWidth(150)
+    parent.button_add_region.clicked.connect(parent.clickmethod)
+    parent.button_add_region.setShortcut("R")
+    parent.button_add_region.setToolTip("Use R Key for shortcut")
+    parent.button_add_region.setStatusTip('The first left click sets the value for the next clicks, then draw polygons')
     
+    # NEW CELL
+    parent.button_newcell.setCheckable(True)
+    parent.button_newcell.setMaximumWidth(150)
+    parent.button_newcell.setShortcut("N")
+    parent.button_newcell.setToolTip("Use N Key for shortcut")
+    parent.button_newcell.clicked.connect(parent.ClickNewCell)
+    parent.button_newcell.setStatusTip('Use the left click to produce a polygon with a new cell value')
     
-#        configuration of all the buttons, some buttons just need to be toggled
-#        meaning that they need just to be clicked and the function associated 
-#        to the button is called and executed. Other buttons are checkable 
-#        meaning that until the user has not finished to use the function
-#        connected to the button, this button stays active (or Checked)
+    # NEXT FRAME (TIME AXIS)
+    parent.button_nextframe.toggle()
+    parent.button_nextframe.pressed.connect(parent.ChangeNextFrame)
+    parent.button_nextframe.setToolTip("Use right arrow key for shortcut")
+    parent.button_nextframe.setMaximumWidth(150)
+    parent.button_nextframe.setShortcut(Qt.Key_Right)
     
+    # if there is only one picture than this button is disabled
+    if np.all(parent.nextframe == 0):
+        parent.button_nextframe.setEnabled(False)    
     
-#       hide / show cnn buttons
-        parent.button_hide_show.setCheckable(True)    
-        parent.button_hide_show.setMaximumWidth(150)
-        parent.button_hide_show.clicked.connect(parent.ShowHideCNNbuttons)
-        # parent.button_hide_show.setShortcut("R")
-        # parent.button_hide_show.setToolTip("Use R Key for shortcut")
-        parent.button_hide_show.setStatusTip('Press to hide and show neural network buttons')
-        
-        
+    # PREVIOUS FRAME (TIME AXIS)
+    parent.button_previousframe.setEnabled(False)
+    parent.button_previousframe.toggle()
+    parent.button_previousframe.pressed.connect(parent.ChangePreviousFrame)
+    parent.button_previousframe.setToolTip("Use left arrow key for shortcut")
+    parent.button_previousframe.setMaximumWidth(150)
+    parent.button_previousframe.setShortcut(Qt.Key_Left)
+    parent.button_previousframe.move(100,100)
+        
+    # ZOOM
+    parent.button_zoom = QPushButton()
+    parent.button_zoom.clicked.connect(parent.ZoomTlbar)
+    parent.button_zoom.setIcon(QtGui.QIcon('./icons/ZoomIcon.png'))
+    parent.button_zoom.setMaximumWidth(30)
+    parent.button_zoom.setMaximumHeight(30)
+    parent.button_zoom.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
+    parent.button_zoom.setCheckable(True)
+    parent.button_zoom.setShortcut("Z")
+    parent.button_zoom.setToolTip("Use Z Key for shortcut")
+    parent.buttonlist.append(parent.button_zoom)
     
-#       ADD REGION
-        parent.button_add_region.setCheckable(True)
-        parent.button_add_region.setMaximumWidth(150)
-        parent.button_add_region.clicked.connect(parent.clickmethod)
-#        the connect function calls clickmethod when the button is clicked.
-        parent.button_add_region.setShortcut("R")
-        parent.button_add_region.setToolTip("Use R Key for shortcut")
-        parent.button_add_region.setStatusTip('The first left click sets the value for the next clicks, then draw polygons')
-        
-        
-#        NEW CELL
-        parent.button_newcell.setCheckable(True)
-        parent.button_newcell.setMaximumWidth(150)
-        parent.button_newcell.setShortcut("N")
-        parent.button_newcell.setToolTip("Use N Key for shortcut")
-#        when the button is pushed the ClickNewCell method is called.
-        parent.button_newcell.clicked.connect(parent.ClickNewCell)
-        parent.button_newcell.setStatusTip('Use the left click to produce a polygon with a new cell value')
-        
-#        NEXT FRAME (TIME AXIS)
-#        this button is used to navigate in the time axis, to show the t+1 frame        
-#        parent.button_nextframe.setCheckable(True)
-        parent.button_nextframe.toggle()
-        parent.button_nextframe.pressed.connect(parent.ChangeNextFrame)
-#        parent.button_nextframe.released.connect(parent.test2)
-        parent.button_nextframe.setToolTip("Use right arrow key for shortcut")
-        parent.button_nextframe.setMaximumWidth(150)
-        parent.button_nextframe.setShortcut(Qt.Key_Right)
-        # if there is only one picture than this button is disabled
-        if np.all(parent.nextframe == 0):
-            parent.button_nextframe.setEnabled(False)
-#        parent.buttonlist.append(parent.button_nextframe)
-#        parent.button_nextframe.setShortcutAutoRepeat(False)
-        
-        
-#        PREVIOUS FRAME (TIME AXIS)
-        
-#        this button is used to load the previous frame in the time axis
-#        parent.button_previousframe.setCheckable(True)
-        parent.button_previousframe.setEnabled(False)
-        parent.button_previousframe.toggle()
-        parent.button_previousframe.pressed.connect(parent.ChangePreviousFrame)
-        parent.button_previousframe.setToolTip("Use left arrow key for shortcut")
-        parent.button_previousframe.setMaximumWidth(150)
-        parent.button_previousframe.setShortcut(Qt.Key_Left)
-        parent.button_previousframe.move(100,100)
-        
-        
-        
-#        Initializing the buttons appearing in the navigation toolbar of the 
-#        matplotlib library and connect them to the original functions already
-#        implemented in the navigation toolbar
-        
-#        ZOOM
-        parent.button_zoom = QPushButton()
-        parent.button_zoom.clicked.connect(parent.ZoomTlbar)
-        parent.button_zoom.setIcon(QtGui.QIcon('./icons/ZoomIcon.png'))
-        parent.button_zoom.setMaximumWidth(30)
-        parent.button_zoom.setMaximumHeight(30)
-        parent.button_zoom.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
-        parent.button_zoom.setCheckable(True)
-        parent.button_zoom.setShortcut("Z")
-        parent.button_zoom.setToolTip("Use Z Key for shortcut")
-        parent.buttonlist.append(parent.button_zoom)
-        
-#        HOME
-        parent.button_home = QPushButton()
-        parent.button_home.clicked.connect(parent.HomeTlbar)
-        parent.button_home.setIcon(QtGui.QIcon('./icons/HomeIcon.png'))
-        parent.button_home.setMaximumWidth(30)
-        parent.button_home.setMaximumHeight(30)
-        parent.button_home.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
-        parent.button_home.setShortcut("H")
-        parent.button_home.setToolTip("Use H Key for shortcut")
-        parent.buttonlist.append(parent.button_home)
-        
-#        PREVIOUS SCALE (ZOOM SCALE)
-        parent.button_back = QPushButton()
-        parent.button_back.clicked.connect(parent.BackTlbar)
-        parent.button_back.setIcon(QtGui.QIcon('./icons/LeftArrowIcon.png'))
-        parent.button_back.setMaximumWidth(30)
-        parent.button_back.setMaximumHeight(30)
-        parent.button_back.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
-        parent.buttonlist.append(parent.button_back)
-        
-        
-#        NEXT SCALE (ZOOM SCALE)
-        parent.button_forward = QPushButton()
-        parent.button_forward.clicked.connect(parent.ForwardTlbar)
-        parent.button_forward.setIcon(QtGui.QIcon('./icons/RightArrowIcon.png'))
-        parent.button_forward.setMaximumWidth(30)
-        parent.button_forward.setMaximumHeight(30)
-        parent.button_forward.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
-        parent.buttonlist.append(parent.button_forward)
-        
-#        PAN 
-        parent.button_pan = QPushButton()
-        parent.button_pan.clicked.connect(parent.PanTlbar)
-        parent.button_pan.setIcon(QtGui.QIcon('./icons/MoveArrowsIcon.png'))
-        parent.button_pan.setMaximumWidth(30)
-        parent.button_pan.setMaximumHeight(30)
-        parent.button_pan.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
-        parent.button_pan.setCheckable(True)
-        parent.button_pan.setShortcut("P")
-        parent.button_pan.setToolTip("Use P Key for shortcut")
-        parent.buttonlist.append(parent.button_pan)
-        
-        
-#        SAVE
-#        this button is used to save the current mask
-        parent.button_savemask.toggle()
-        parent.button_savemask.setEnabled(True)
-        parent.button_savemask.clicked.connect(parent.ButtonSaveMask)
-        parent.button_savemask.setToolTip("Use S Key for shortcut")
-        parent.button_savemask.setMaximumWidth(150)
-        parent.button_savemask.setShortcut("S")
-        parent.button_savemask.setStatusTip('Save the mask')
-
-
-#       BRUSH 
-#        parent.button_drawmouse.setEnabled(True)
-        parent.button_drawmouse.setCheckable(True)
-        parent.button_drawmouse.clicked.connect(parent.MouseDraw)
-        parent.button_drawmouse.setToolTip("Use B Key for shortcut")
-        parent.button_drawmouse.setShortcut("B")
-        parent.button_drawmouse.setMaximumWidth(150)
-        parent.button_drawmouse.setStatusTip('Right click to select cell value and then left click and drag to draw')
-        
-        
-#        ERASER
-        parent.button_eraser.setCheckable(True)
-        parent.button_eraser.clicked.connect(parent.MouseDraw)
-        parent.button_eraser.setToolTip("Use E Key for shortcut")
-        parent.button_eraser.setShortcut("E")
-        parent.button_eraser.setMaximumWidth(150)
-        parent.button_eraser.setStatusTip('Right click and drag to set values to 0')
-        
-#        EXCHANGE CELL VALUES
-        parent.button_exval.toggle()
-        parent.button_exval.setEnabled(True)
-        parent.button_exval.clicked.connect(parent.DialogBoxECV)
-        parent.button_exval.setMaximumWidth(150)
-        parent.button_exval.setStatusTip('Exchange values between two cells')
-
-#        CHANGE CELL VALUE
-        parent.button_changecellvalue.setCheckable(True)
-        parent.button_changecellvalue.setEnabled(True)
-        parent.button_changecellvalue.clicked.connect(parent.ChangeOneValue)
-        parent.button_changecellvalue.setMaximumWidth(150)
-        parent.button_changecellvalue.setStatusTip('Change value of one cell')
-        parent.button_changecellvalue.setToolTip('Use left click to select one cell and enter a new value')
-        
-        
-        
-#        THRESHOLD THE PREDICTION CHECKBOX
-        parent.button_threshold.setEnabled(False)
-        parent.button_threshold.stateChanged.connect(parent.ThresholdBoxCheck)
-        
-#        TEXT BOX FOR ENTERING THRESHOLD VALUE
-        parent.button_SetThreshold = QLineEdit()
-        parent.button_SetThreshold.setPlaceholderText('Enter a threshold value')
-        parent.button_SetThreshold.setValidator(QtGui.QDoubleValidator())
-        parent.button_SetThreshold.setMaximumWidth(150)
-        parent.button_SetThreshold.returnPressed.connect(parent.ThresholdPrediction)
-        parent.button_SetThreshold.setEnabled(False)
-        
-
+    # HOME
+    parent.button_home = QPushButton()
+    parent.button_home.clicked.connect(parent.HomeTlbar)
+    parent.button_home.setIcon(QtGui.QIcon('./icons/HomeIcon.png'))
+    parent.button_home.setMaximumWidth(30)
+    parent.button_home.setMaximumHeight(30)
+    parent.button_home.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
+    parent.button_home.setShortcut("H")
+    parent.button_home.setToolTip("Use H Key for shortcut")
+    parent.buttonlist.append(parent.button_home)
+    
+    # PREVIOUS SCALE (ZOOM SCALE)
+    parent.button_back = QPushButton()
+    parent.button_back.clicked.connect(parent.BackTlbar)
+    parent.button_back.setIcon(QtGui.QIcon('./icons/LeftArrowIcon.png'))
+    parent.button_back.setMaximumWidth(30)
+    parent.button_back.setMaximumHeight(30)
+    parent.button_back.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
+    parent.buttonlist.append(parent.button_back)
+    
+    # NEXT SCALE (ZOOM SCALE)
+    parent.button_forward = QPushButton()
+    parent.button_forward.clicked.connect(parent.ForwardTlbar)
+    parent.button_forward.setIcon(QtGui.QIcon('./icons/RightArrowIcon.png'))
+    parent.button_forward.setMaximumWidth(30)
+    parent.button_forward.setMaximumHeight(30)
+    parent.button_forward.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
+    parent.buttonlist.append(parent.button_forward)
+    
+    # PAN 
+    parent.button_pan = QPushButton()
+    parent.button_pan.clicked.connect(parent.PanTlbar)
+    parent.button_pan.setIcon(QtGui.QIcon('./icons/MoveArrowsIcon.png'))
+    parent.button_pan.setMaximumWidth(30)
+    parent.button_pan.setMaximumHeight(30)
+    parent.button_pan.setStyleSheet("border: 0px" "QPushButton:hover { background-color: blue }" )
+    parent.button_pan.setCheckable(True)
+    parent.button_pan.setShortcut("P")
+    parent.button_pan.setToolTip("Use P Key for shortcut")
+    parent.buttonlist.append(parent.button_pan)
+    
+    # SAVE
+    parent.button_savemask.toggle()
+    parent.button_savemask.setEnabled(True)
+    parent.button_savemask.clicked.connect(parent.ButtonSaveMask)
+    parent.button_savemask.setToolTip("Use S Key for shortcut")
+    parent.button_savemask.setMaximumWidth(150)
+    parent.button_savemask.setShortcut("S")
+    parent.button_savemask.setStatusTip('Save the mask')
 
-#        SAVE BUTTON FOR THE THRESHOLDED PREDICTION
-        parent.button_savethresholdmask = QPushButton('Save Threshold')
-        parent.button_savethresholdmask.toggle()
-        parent.button_savethresholdmask.setEnabled(False)
-        parent.button_savethresholdmask.clicked.connect(parent.ButtonSaveThresholdMask)
-        parent.button_savethresholdmask.setMaximumWidth(150)
-        parent.button_savethresholdmask.setStatusTip('Save the thresholded prediction')
-        
-        
-#        SEGMENT THE OUTPUT OF THE THRESHOLD
-        parent.button_segment.setEnabled(False)
-        parent.button_segment.stateChanged.connect(parent.SegmentBoxCheck)
-        
-#        TEXT BOX FOR THE PARAMETERS OF THE SEGMENTATION
-        parent.button_SetSegmentation = QLineEdit()
-        parent.button_SetSegmentation.setPlaceholderText('Enter param for seg')
-        parent.button_SetSegmentation.setValidator(QtGui.QIntValidator())
-        parent.button_SetSegmentation.setMaximumWidth(80)
-        parent.button_SetSegmentation.returnPressed.connect(parent.SegmentThresholdedPredMask)
-        parent.button_SetSegmentation.setEnabled(False)
-        parent.button_SetSegmentation.setText('10')
-        
+    # BRUSH 
+    parent.button_drawmouse.setCheckable(True)
+    parent.button_drawmouse.clicked.connect(parent.MouseDraw)
+    parent.button_drawmouse.setToolTip("Use B Key for shortcut")
+    parent.button_drawmouse.setShortcut("B")
+    parent.button_drawmouse.setMaximumWidth(150)
+    parent.button_drawmouse.setStatusTip('Right click to select cell value and then left click and drag to draw')
+    
+    # ERASER
+    parent.button_eraser.setCheckable(True)
+    parent.button_eraser.clicked.connect(parent.MouseDraw)
+    parent.button_eraser.setToolTip("Use E Key for shortcut")
+    parent.button_eraser.setShortcut("E")
+    parent.button_eraser.setMaximumWidth(150)
+    parent.button_eraser.setStatusTip('Right click and drag to set values to 0')
+    
+    # EXCHANGE CELL VALUES
+    parent.button_exval.toggle()
+    parent.button_exval.setEnabled(True)
+    parent.button_exval.clicked.connect(parent.DialogBoxECV)
+    parent.button_exval.setMaximumWidth(150)
+    parent.button_exval.setStatusTip('Exchange values between two cells')
 
-#        SAVE BUTTON FOR THE SEGMENTATION OF THE THRESHOLDED PREDICTION
-        parent.button_savesegmask = QPushButton('Save Seg')
-        parent.button_savesegmask.toggle()
-        parent.button_savesegmask.setEnabled(False)
-        parent.button_savesegmask.clicked.connect(parent.ButtonSaveSegMask)
-        parent.button_savesegmask.setMaximumWidth(150)
-        parent.button_savesegmask.setStatusTip('Save the segmented thresholded prediction')
-        
-        
-        
-#        MAKE THE CELL CORRESPONDANCE
-        parent.button_cellcorespondance.setEnabled(False)
-        parent.button_cellcorespondance.setCheckable(True)
-        parent.button_cellcorespondance.clicked.connect(parent.CellCorrespActivation)
-        parent.button_cellcorespondance.setMaximumWidth(150)
-        parent.button_cellcorespondance.setStatusTip('Do the cell correspondance with the previous time frame')
-        
-        
-#        EXTRACT FLUORESCENCE IN DIFFERENT CHANNELS
-        
-        parent.button_extractfluorescence.setEnabled(False)
-        parent.button_extractfluorescence.toggle()
-        parent.button_extractfluorescence.clicked.connect(parent.ButtonFluo)
-        parent.button_extractfluorescence.setMaximumWidth(150)
-        parent.button_extractfluorescence.setStatusTip('Extract the total intensity, area and variance of the cells in the different channels')
-        
-        
-#        SHOW THE VALUES OF THE CELLS
-#        parent.button_showval.checkStateSet(False)
-        parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersCurr)
-        parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersPrev)
-        parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersNext)
-        parent.button_showval.setShortcut('V')
-        parent.button_showval.setToolTip("Use V Key for shortcut")
-        
-        
-#        HIDE/SHOW THE MASK
-        parent.button_hidemask.stateChanged.connect(parent.m.HideMask)
-        
-        
-#        CHANGE TIME INDEX
-        parent.button_timeindex = QLineEdit()
-        parent.button_timeindex.setPlaceholderText('Time index 0-{}'.format(parent.reader.sizet-1))
-        parent.button_timeindex.setValidator(QtGui.QIntValidator(0,int(parent.reader.sizet-1)))
-        parent.button_timeindex.returnPressed.connect(parent.ChangeTimeFrame)
-        parent.button_timeindex.setMaximumWidth(150)
-       
-        parent.buttonlist.append(parent.button_timeindex)
-        
-        
-        
-#        FIELDS OF VIEW BUTTON
-#        Create a widget that displays the lists of different Fields of view.
-        parent.button_fov = QComboBox()
-#        create a list of different FOV which will be dispalyed in the widget.
-        list_fov = []
-        for i in range(0, parent.reader.Npos):
-            list_fov.append("Field of View " + str(i+1))
-        parent.button_fov.addItems(list_fov)
-        parent.button_fov.setMaximumWidth(150)
-        parent.buttonlist.append(parent.button_fov)
-#        connects the selection of one option in the ComboBox to the selectFOV 
-#        function.   
-        parent.button_fov.activated.connect(parent.SelectFov)
-        
-        
-                
-#        CHANGE CHANNEL BUTTON
-#        Create a widget that displays a list of the different channels.
-#        in order to switch between them
-        parent.button_channel = QComboBox()
-        parent.button_channel.addItems(parent.reader.channel_names)
-        parent.button_channel.setMaximumWidth(150)
-        parent.buttonlist.append(parent.button_channel)
-#        connects the selection of one option in the ComboBox to the selectFOV 
-#        function.   
-        parent.button_channel.activated.connect(parent.SelectChannel)
-        
-        
-        
-        
-        
-        
-#        NEURAL NETWORK BUTTON
-        parent.button_cnn.setCheckable(True)
-        parent.button_cnn.pressed.connect(parent.LaunchBatchPrediction)
-        parent.button_cnn.setToolTip("Launches the CNN on a range of images")
-        parent.button_cnn.setMaximumWidth(150)
-        parent.EnableCNNButtons()
+    # CHANGE CELL VALUE
+    parent.button_changecellvalue.setCheckable(True)
+    parent.button_changecellvalue.setEnabled(True)
+    parent.button_changecellvalue.clicked.connect(parent.ChangeOneValue)
+    parent.button_changecellvalue.setMaximumWidth(150)
+    parent.button_changecellvalue.setStatusTip('Change value of one cell')
+    parent.button_changecellvalue.setToolTip('Use left click to select one cell and enter a new value')
+        
+    # THRESHOLD THE PREDICTION CHECKBOX
+    parent.button_threshold.setEnabled(False)
+    parent.button_threshold.stateChanged.connect(parent.ThresholdBoxCheck)
+    
+    # TEXT BOX FOR ENTERING THRESHOLD VALUE
+    parent.button_SetThreshold = QLineEdit()
+    parent.button_SetThreshold.setPlaceholderText('Enter a threshold value')
+    parent.button_SetThreshold.setValidator(QtGui.QDoubleValidator())
+    parent.button_SetThreshold.setMaximumWidth(150)
+    parent.button_SetThreshold.returnPressed.connect(parent.ThresholdPrediction)
+    parent.button_SetThreshold.setEnabled(False)
+    
+    # SAVE BUTTON FOR THE THRESHOLDED PREDICTION
+    parent.button_savethresholdmask = QPushButton('Save Threshold')
+    parent.button_savethresholdmask.toggle()
+    parent.button_savethresholdmask.setEnabled(False)
+    parent.button_savethresholdmask.clicked.connect(parent.ButtonSaveThresholdMask)
+    parent.button_savethresholdmask.setMaximumWidth(150)
+    parent.button_savethresholdmask.setStatusTip('Save the thresholded prediction')
+    
+    # SEGMENT THE OUTPUT OF THE THRESHOLD
+    parent.button_segment.setEnabled(False)
+    parent.button_segment.stateChanged.connect(parent.SegmentBoxCheck)
+    
+    # TEXT BOX FOR THE PARAMETERS OF THE SEGMENTATION
+    parent.button_SetSegmentation = QLineEdit()
+    parent.button_SetSegmentation.setPlaceholderText('Enter param for seg')
+    parent.button_SetSegmentation.setValidator(QtGui.QIntValidator())
+    parent.button_SetSegmentation.setMaximumWidth(80)
+    parent.button_SetSegmentation.returnPressed.connect(parent.SegmentThresholdedPredMask)
+    parent.button_SetSegmentation.setEnabled(False)
+    parent.button_SetSegmentation.setText('10')
+    
+    # SAVE BUTTON FOR THE SEGMENTATION OF THE THRESHOLDED PREDICTION
+    parent.button_savesegmask = QPushButton('Save Seg')
+    parent.button_savesegmask.toggle()
+    parent.button_savesegmask.setEnabled(False)
+    parent.button_savesegmask.clicked.connect(parent.ButtonSaveSegMask)
+    parent.button_savesegmask.setMaximumWidth(150)
+    parent.button_savesegmask.setStatusTip('Save the segmented thresholded prediction')
+        
+    # MAKE THE CELL CORRESPONDANCE
+    parent.button_cellcorespondance.setEnabled(False)
+    parent.button_cellcorespondance.setCheckable(True)
+    parent.button_cellcorespondance.clicked.connect(parent.CellCorrespActivation)
+    parent.button_cellcorespondance.setMaximumWidth(150)
+    parent.button_cellcorespondance.setStatusTip('Do the cell correspondance with the previous time frame')
+    
+    # EXTRACT FLUORESCENCE IN DIFFERENT CHANNELS    
+    parent.button_extractfluorescence.setEnabled(False)
+    parent.button_extractfluorescence.toggle()
+    parent.button_extractfluorescence.clicked.connect(parent.ButtonFluo)
+    parent.button_extractfluorescence.setMaximumWidth(150)
+    parent.button_extractfluorescence.setStatusTip('Extract the total intensity, area and variance of the cells in the different channels')
+    
+    # SHOW THE VALUES OF THE CELLS
+    parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersCurr)
+    parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersPrev)
+    parent.button_showval.stateChanged.connect(parent.m.ShowCellNumbersNext)
+    parent.button_showval.setShortcut('V')
+    parent.button_showval.setToolTip("Use V Key for shortcut")
+        
+    # HIDE/SHOW THE MASK
+    parent.button_hidemask.stateChanged.connect(parent.m.HideMask)    
+    
+    # CHANGE TIME INDEX
+    parent.button_timeindex = QLineEdit()
+    parent.button_timeindex.setPlaceholderText('Time index 0-{}'.format(parent.reader.sizet-1))
+    parent.button_timeindex.setValidator(QtGui.QIntValidator(0,int(parent.reader.sizet-1)))
+    parent.button_timeindex.returnPressed.connect(parent.ChangeTimeFrame)
+    parent.button_timeindex.setMaximumWidth(150)
+    parent.buttonlist.append(parent.button_timeindex)
+    
+    # FIELDS OF VIEW BUTTON
+    parent.button_fov = QComboBox()
+    list_fov = []
+    for i in range(0, parent.reader.Npos):
+        list_fov.append("Field of View " + str(i+1))
+    parent.button_fov.addItems(list_fov)
+    parent.button_fov.setMaximumWidth(150)
+    parent.buttonlist.append(parent.button_fov)
+    parent.button_fov.activated.connect(parent.SelectFov)
+            
+    # CHANGE CHANNEL BUTTON
+    parent.button_channel = QComboBox()
+    parent.button_channel.addItems(parent.reader.channel_names)
+    parent.button_channel.setMaximumWidth(150)
+    parent.buttonlist.append(parent.button_channel)
+    parent.button_channel.activated.connect(parent.SelectChannel)
+        
+    # NEURAL NETWORK BUTTON
+    parent.button_cnn.setCheckable(True)
+    parent.button_cnn.pressed.connect(parent.LaunchBatchPrediction)
+    parent.button_cnn.setToolTip("Launches the CNN on a range of images")
+    parent.button_cnn.setMaximumWidth(150)
+    parent.EnableCNNButtons()